Journal: Heliyon

Article Title: Effects of chronic treatment with metformin on brain glucose hypometabolism and central insulin actions in transgenic mice with tauopathy

doi: 10.1016/j.heliyon.2024.e35752

Figure Lengend Snippet: Identification of primers used in the different gene expression assays.

Article Snippet: Anti IRS1 , Rabbit , BioRad.

Techniques: Gene Expression, TaqMan Assay

Journal: Heliyon

Article Title: Effects of chronic treatment with metformin on brain glucose hypometabolism and central insulin actions in transgenic mice with tauopathy

doi: 10.1016/j.heliyon.2024.e35752

Figure Lengend Snippet: Antibodies used for protein detection by Western Blot.

Article Snippet: Anti IRS1 , Rabbit , BioRad.

Techniques: Western Blot

Journal: Heliyon

Article Title: Effects of chronic treatment with metformin on brain glucose hypometabolism and central insulin actions in transgenic mice with tauopathy

doi: 10.1016/j.heliyon.2024.e35752

Figure Lengend Snippet: Effect of metformin on the expression of the total (A, B and C), phosphorylated (D, E and F) and active (G, H and I) forms of IR, IRS1 and PI3K, respectively, in the frontal cortex of Tau-VLW transgenic mice. 9-months-old Tau mice were treated daily with metformin for another 9 months and then the expression of the indicated proteins was measured by Western blot as described in Experimental procedures. Total and phosphorylated forms were normalized by β-actin (representative bands are showed in ). Phosphorylated forms normalized to the total forms are represented as active forms. Data are represented as means ± SEM (n = 4–5). Student's unpaired t -test was used to analyse significant differences. *p < 0.05, **p < 0.01. The full-length blot is provided in supplementary material as jpg and jpg.

Article Snippet: Anti IRS1 , Rabbit , BioRad.

Techniques: Expressing, Transgenic Assay, Western Blot

Journal: Frontiers in Pharmacology

Article Title: Edgeworthia gardneri (Wall.) Meisn. Water Extract Ameliorates Palmitate Induced Insulin Resistance by Regulating IRS1/GSK3β/FoxO1 Signaling Pathway in Human HepG2 Hepatocytes

doi: 10.3389/fphar.2019.01666

Figure Lengend Snippet: WEE affected the molecular components of IRS1/GSK-3β/FoxO1 pathways in PA treated HepG2 cells. HepG2 cells were incubated with normal glucose (5.5 mM) or high glucose (30 mM) plus PA (0.3 mM) in the absence or presence of WEE (200 and 300 μg/ml) for 24 h and followed by being stimulated with insulin (100 nM) for 20 min. Then, the phosphorylation and total levels of IRβ, IRS-1, GSK3β, CREB, c-Jun, FoxO1, Akt, p38, JNK, and ERK were detected by Western blotting. Bar graphs show the relative expression of indicated proteins. All proteins were normalized for β-actin levels. Data presented in bar charts are mean ± SEM values from three independent experiments. Groups are significantly different from the control group at *p < 0.05, **p < 0.01. The groups are significantly different from the IR group at # p < 0.05 and ## p < 0.01 determined by Dunnett’s multiple comparisons test.

Article Snippet: Phospho-IRS1 (Tyr 612, catalog no. PAB12628) was purchased from Abnova (Taiwan, China).

Techniques: Incubation, Western Blot, Expressing, Control